LOCUS       LB_091                  6009 bp    DNA     circular     07-FEB-2013
FEATURES             Location/Qualifiers
     misc_feature    1867..3519
                     /note="luc2"
     misc_feature    3554..3775
                     /note="SV40 late poly(A) signal"
     misc_feature    3843..3862
                     /note="RV primer 4 binding region"
     misc_feature    4891..5751
                     /note="Amp(r)"
     misc_feature    5958..5977
                     /note="RVprimer3 binding region"
     PCR_primer      complement(1845..1864)
                     /dnas_title="TGGCTTTACCAACAGTACCG"
                     /pair=""
                     /primer="TGGCTTTACCAACAGTACCG"
                     /current=0
     PCR_primer      20..52
                     /note=""
                     /current=0
     misc_feature    19..144
                     /dnas_title="attR1"
                     /vntifkey="21"
                     /label=attR1
     mRNA            19..1724
                     /note="gatewayLR cassette"
     misc_feature    1254..1559
                     /note="ccdB survival"
     misc_feature    1600..1724
                     /dnas_title="attR2"
                     /vntifkey="21"
                     /label=attR2
     PCR_primer      complement(1692..1724)
                     /dnas_title="ACCACTTTGTACAAGAAAGCTGAACGAGAAACG"
                     /pair="gatewayLR-rev"
                     /primer="ACCACTTTGTACAAGAAAGCTGAACGAGAAACG"
                     /current=0
     PCR_primer      5958..5977
                     /dnas_title="CTAGCAAAATAGGCTGTCCC"
                     /pair=""
                     /primer="CTAGCAAAATAGGCTGTCCC"
                     /current=1
     misc_feature    1725..1832
                     /note="MCS2"
     misc_feature    1726..1820
                     /note="SCP1"
     source          1..6009
                     /dnas_title="LB_091"
ORIGIN      
        1 GGCCTAACTG GCCGGTACCa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
       61 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      121 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
      241 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      301 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      361 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      421 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      481 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      541 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      601 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      661 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      721 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      781 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      841 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      901 ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
      961 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     1021 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     1081 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     1141 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     1201 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     1261 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     1321 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     1381 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     1441 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     1501 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     1561 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     1621 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     1681 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtAGATCT CCCTAGGGTA
     1741 CTTATATAAG GGGGTGGGGG CGCGTTCGTC CTCAGTCGCG ATCGAACACT CGAGCCGAGC
     1801 AGACGTGCCT ACGGACCGGG CTAGCgacct aAAGCTTGGC AATCCGGTAC TGTTGGTAAA
     1861 GCCACCATGG AAGATGCCAA AAACATTAAG AAGGGCCCAG CGCCATTCTA CCCACTCGAA
     1921 GACGGGACCG CCGGCGAGCA GCTGCACAAA GCCATGAAGC GCTACGCCCT GGTGCCCGGC
     1981 ACCATCGCCT TTACCGACGC ACATATCGAG GTGGACATTA CCTACGCCGA GTACTTCGAG
     2041 ATGAGCGTTC GGCTGGCAGA AGCTATGAAG CGCTATGGGC TGAATACAAA CCATCGGATC
     2101 GTGGTGTGCA GCGAGAATAG CTTGCAGTTC TTCATGCCCG TGTTGGGTGC CCTGTTCATC
     2161 GGTGTGGCTG TGGCCCCAGC TAACGACATC TACAACGAGC GCGAGCTGCT GAACAGCATG
     2221 GGCATCAGCC AGCCCACCGT CGTATTCGTG AGCAAGAAAG GGCTGCAAAA GATCCTCAAC
     2281 GTGCAAAAGA AGCTACCGAT CATACAAAAG ATCATCATCA TGGATAGCAA GACCGACTAC
     2341 CAGGGCTTCC AAAGCATGTA CACCTTCGTG ACTTCCCATT TGCCACCCGG CTTCAACGAG
     2401 TACGACTTCG TGCCCGAGAG CTTCGACCGG GACAAAACCA TCGCCCTGAT CATGAACAGT
     2461 AGTGGCAGTA CCGGATTGCC CAAGGGCGTA GCCCTACCGC ACCGCACCGC TTGTGTCCGA
     2521 TTCAGTCATG CCCGCGACCC CATCTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTC
     2581 AGCGTGGTGC CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC
     2641 GGCTTTCGGG TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA
     2701 GACTATAAGA TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTTCTT CGCTAAGAGC
     2761 ACTCTCATCG ACAAGTACGA CCTAAGCAAC TTGCACGAGA TCGCCAGCGG CGGGGCGCCG
     2821 CTCAGCAAGG AGGTAGGTGA GGCCGTGGCC AAACGCTTCC ACCTACCAGG CATCCGCCAG
     2881 GGCTACGGCC TGACAGAAAC AACCAGCGCC ATTCTGATCA CCCCCGAAGG GGACGACAAG
     2941 CCTGGCGCAG TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTGGA CTTGGACACC
     3001 GGTAAGACAC TGGGTGTGAA CCAGCGCGGC GAGCTGTGCG TCCGTGGCCC CATGATCATG
     3061 AGCGGCTACG TTAACAACCC CGAGGCTACA AACGCTCTCA TCGACAAGGA CGGCTGGCTG
     3121 CACAGCGGCG ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG
     3181 AAGAGCCTGA TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GAGCATCCTG
     3241 CTGCAACACC CCAACATCTT CGACGCCGGG GTCGCCGGCC TGCCCGACGA CGATGCCGGC
     3301 GAGCTGCCCG CCGCAGTCGT CGTGCTGGAA CACGGTAAAA CCATGACCGA GAAGGAGATC
     3361 GTGGACTATG TGGCCAGCCA GGTTACAACC GCCAAGAAGC TGCGCGGTGG TGTTGTGTTC
     3421 GTGGACGAGG TGCCTAAAGG ACTGACCGGC AAGTTGGACG CCCGCAAGAT CCGCGAGATT
     3481 CTCATTAAGG CCAAGAAGGG CGGCAAGATC GCCGTGTAAT AATTCTAGAG TCGGGGCGGC
     3541 CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
     3601 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
     3661 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
     3721 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC
     3781 GATAAGGATC CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG
     3841 GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT
     3901 AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG
     3961 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT
     4021 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA
     4081 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
     4141 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
     4201 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
     4261 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
     4321 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG
     4381 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
     4441 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA
     4501 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT
     4561 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
     4621 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA
     4681 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
     4741 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT
     4801 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA
     4861 GCGGCCGCAA ATGCTAAACC ACTGCAGTGG TTACCAGTGC TTGATCAGTG AGGCACCGAT
     4921 CTCAGCGATC TGCCTATTTC GTTCGTCCAT AGTGGCCTGA CTCCCCGTCG TGTAGATCAC
     4981 TACGATTCGT GAGGGCTTAC CATCAGGCCC CAGCGCAGCA ATGATGCCGC GAGAGCCGCG
     5041 TTCACCGGCC CCCGATTTGT CAGCAATGAA CCAGCCAGCA GGGAGGGCCG AGCGAAGAAG
     5101 TGGTCCTGCT ACTTTGTCCG CCTCCATCCA GTCTATGAGC TGCTGTCGTG ATGCTAGAGT
     5161 AAGAAGTTCG CCAGTGAGTA GTTTCCGAAG AGTTGTGGCC ATTGCTACTG GCATCGTGGT
     5221 ATCACGCTCG TCGTTCGGTA TGGCTTCGTT CAACTCTGGT TCCCAGCGGT CAAGCCGGGT
     5281 CACATGATCA CCCATATTAT GAAGAAATGC AGTCAGCTCC TTAGGGCCTC CGATCGTTGT
     5341 CAGAAGTAAG TTGGCCGCGG TGTTGTCGCT CATGGTAATG GCAGCACTAC ACAATTCTCT
     5401 TACCGTCATG CCATCCGTAA GATGCTTTTC CGTGACCGGC GAGTACTCAA CCAAGTCGTT
     5461 TTGTGAGTAG TGTATACGGC GACCAAGCTG CTCTTGCCCG GCGTCTATAC GGGACAACAC
     5521 CGCGCCACAT AGCAGTACTT TGAAAGTGCT CATCATCGGG AATCGTTCTT CGGGGCGGAA
     5581 AGACTCAAGG ATCTTGCCGC TATTGAGATC CAGTTCGATA TAGCCCACTC TTGCACCCAG
     5641 TTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCGGGG TGTGCAAAAA CAGGCAAGCA
     5701 AAATGCCGCA AAGAAGGGAA TGAGTGCGAC ACGAAAATGT TGGATGCTCA TACTCGTCCT
     5761 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTACTAGTAC GTCTCTCAAG GATAAGTAAG
     5821 TAATATTAAG GTACGGGAGG TATTGGACAG GCCGCAATAA AATATCTTTA TTTTCATTAC
     5881 ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC ATACGCTCTC CATCAAAACA
     5941 AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA
     6001 CATTTCTCT
//

