LOCUS pGL3\Promoter\sg 4080 bp DNA circular 12-NOV-2008 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|498754724| COMMENT VNTDBDATE|498754762| COMMENT LSOWNER| COMMENT VNTNAME|pGL3 Promoter sgGFP| COMMENT VNTAUTHORNAME|Jenuwein| FEATURES Location/Qualifiers rep_origin 5007..5462 /dnas_title="f1 origin" /vntifkey="33" /label=f1 origin misc_marker complement(4015..4875) /dnas_title="Beta-lactamase gene (Ampr)" /vntifkey="22" /label=Beta-lactamase gene (Ampr) rep_origin 3253..3253 /dnas_title="ColE1-derived plasmid replication origin" /vntifkey="33" /label=ColE1-derived plasmid replication origin primer_bind 2996..3015 /dnas_title="RVprimer4 binding site" /vntifkey="28" /label=RVprimer4 binding site polyA_signal 2710..2931 /dnas_title="SV40 late poly (A) signal" /vntifkey="25" /label=SV40 late poly (A) signal misc_feature 2942..2945 /note="BamHI" misc_feature 2946..2950 /note="SalI" gene 1335..1994 /note="CamR" PCR_primer 1227..1248 /dnas_title="GCGGCCGCATTAGGCACCCCAG" /pair="" /primer="GCGGCCGCATTAGGCACCCCAG" /current=0 misc_feature 2336..2641 /note="ccdB survival" PCR_primer complement(2653..2675) /dnas_title="CTGCAGACTGGCTGTGTATAAGG" /pair="" /primer="CTGCAGACTGGCTGTGTATAAGG" /current=0 primer_bind 103..122 /dnas_title="RVprimer3 binding region" /vntifkey="28" /label=RVprimer3 binding region polyA_signal 1..154 /dnas_title="Synthetic (upstream) poly (A)signal" /vntifkey="25" /label=Synthetic (upstream) poly (A)signal misc_feature 162..193 /dnas_title="MCS" /vntifkey="21" /label=MCS PCR_primer 177..193 /dnas_title="CGCGTGCTAGCCCGGGCTCGAGATCTGCGATC" /pair="" /primer="CGCGTGCTAGCCCGGGCTCGAGATCTGCGATC" /current=0 PCR_primer 193..193 /dnas_title="CTCGAGATCTGCGATCTGCATCTC" /pair="" /primer="CTCGAGATCTGCGATCTGCATCTC" /current=0 PCR_primer 182..192 /dnas_title="GCTAGCCCGGG" /pair="" /primer="GCTAGCCCGGG" /current=0 PCR_primer 185..193 /dnas_title="AGCCCGGGCTCGAGATCTGCGATC" /pair="sgGFP rv seq" /primer="AGCCCGGGCTCGAGATCTGCGATC" /current=0 misc_feature 209..363 /note="DSCP Drosophila synthetic core promoter" misc_feature 483..1202 /note="sgGFP" misc_feature 399..469 /note="MHC intron 16" misc_feature 402..468 /note="intron" misc_feature 392..403 /note="" PCR_primer 425..444 /dnas_title="GGCACACCGAAACGACTAAC" /pair="MHCI-control-03_fw" /primer="GGCACACCGAAACGACTAAC" /current=0 PCR_primer complement(597..620) /dnas_title="CTTCAGGGTAAGTTTTCCGTATGT" /pair="sgGFP_5'-02-rev" /primer="CTTCAGGGTAAGTTTTCCGTATGT" /current=0 PCR_primer complement(544..568) /dnas_title="GAGAACTTGTGGCCGTTAACATCAC" /pair="sgGFP-rev-seq" /primer="GAGAACTTGTGGCCGTTAACATCAC" /current=0 PCR_primer 422..444 /dnas_title="CTAGGCACACCGAAACGACTAAC" /pair="MHCI-control-02_fw" /primer="CTAGGCACACCGAAACGACTAAC" /current=0 misc_feature 471..478 /note="insert" PCR_primer 456..496 /dnas_title="TCCTTTACTTCAGGCCAAAGCTTCATGGCTAGCAAAGG" /pair="QCII-mhc-HindIII-GFP_fw" /primer="TCCTTTACTTCAGGCCAAAGCTTCATGGCTAGCAAAGG" /current=0 PCR_primer complement(456..496) /dnas_title="CCTTTGCTAGCCATGAAGCTTTGGCCTGAAGTAAAGGA" /pair="QCII-mhc-HindIII-GFP_rev" /primer="CCTTTGCTAGCCATGAAGCTTTGGCCTGAAGTAAAGGA" /current=0 misc_feature 473..478 /note="HindIII" misc_feature 476..481 /note="AflII" PCR_primer complement(2653..2714) /dnas_title="GTCTGCTCGAAGCGGCCGGCCGAATTCGTCGACGATATCCTGCAG ACTGGCTGTGTATAAGG" /pair="clone-IF-FseI-rev" /primer="GTCTGCTCGAAGCGGCCGGCCGAATTCGTCGACGATATCCTGCAGACTG GCTGTGTATAAGG" /current=0 PCR_primer 1188..1248 /dnas_title="GAACTGTACAACTGATCTAGAGCATGCACCGGTGATATCGCGGCC GCATTAGGCACCCCAG" /pair="clone-IF-Xba-fw" /primer="GAACTGTACAACTGATCTAGAGCATGCACCGGTGATATCGCGGCCGCAT TAGGCACCCCAG" /current=0 PCR_primer 1209..1223 /dnas_title="GCATGCACCGGTGAT" /pair="PE_read_1_IF-EcoRV-09-fw" /primer="GCATGCACCGGTGAT" /current=0 PCR_primer 1205..1219 /dnas_title="TAGAGCATGCACCGG" /pair="PE_read_1_IF-AgeI-10-fw" /primer="TAGAGCATGCACCGG" /current=0 PCR_primer 1199..1213 /dnas_title="CTGATCTAGAGCATG" /pair="PE_read_1_IF-SphI-11-fw" /primer="CTGATCTAGAGCATG" /current=0 PCR_primer complement(2675..2689) /dnas_title="TCGTCGACGATATCC" /pair="PE_Read_2_IF-PstI-12-rev" /primer="TCGTCGACGATATCC" /current=0 PCR_primer complement(2679..2693) /dnas_title="GAATTCGTCGACGAT" /pair="PE_Read_2_IF-EcoRV-13-rev" /primer="GAATTCGTCGACGAT" /current=0 PCR_primer complement(2683..2697) /dnas_title="GGCCGAATTCGTCGA" /pair="PE_Read_2_IF-SalI-14-rev" /primer="GGCCGAATTCGTCGA" /current=0 misc_feature 266..271 /note="Inr" misc_feature 237..243 /note="TATA" misc_feature 295..300 /note="DPE" PCR_primer complement(4273..4293) /dnas_title="GAACTACTTACTCTAGCTTCC" /pair="AmrR-seq-rev" /primer="GAACTACTTACTCTAGCTTCC" /current=0 PCR_primer complement(2710..2735) /note="GSP1" /current=0 PCR_primer complement(2714..2739) /note="GSP10" /current=0 PCR_primer complement(2700..2720) /note="junction_rev" /current=0 PCR_primer 163..188 /dnas_title="GTACCGAGCTCTTACGCGTGCTAGCC" /pair="DSCP-XhoI-CP_01-fw" /primer="GTACCGAGCTCTTACGCGTGCTAGCC" /current=0 PCR_primer 173..195 /dnas_title="CTTACGCGTGCTAGCCCGGGCTC" /pair="DSCP-XhoI-CP_02-fw" /primer="CTTACGCGTGCTAGCCCGGGCTC" /current=0 PCR_primer 141..168 /dnas_title="CAGAACATTTCTCTATCGATAGGTACCG" /pair="DSCP-XhoI-CP_03-fw" /primer="CAGAACATTTCTCTATCGATAGGTACCG" /current=0 PCR_primer complement(334..363) /dnas_title="GTTTGGTATGCGTCTTGTGATTCAAAGTTG" /pair="DSCP-SbfI-01-rv" /primer="GTTTGGTATGCGTCTTGTGATTCAAAGTTG" /current=0 PCR_primer complement(337..363) /dnas_title="GTTTGGTATGCGTCTTGTGATTCAAAG" /pair="DSCP-SbfI-02-rv" /primer="GTTTGGTATGCGTCTTGTGATTCAAAG" /current=1 source 1..5592 /dnas_title="pGL3 Promoter sgGFP" ORIGIN 1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta 61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc 121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcttacgcg 181 tgctagcccg ggcTCGAGAT CTGCGATCga gctcgcccgg ggatcgagcg cagcggtata 241 aaagggcgcg gggtggctga gagcatcagt tgtgaatgaa tgttcgagcc gagcagacgt 301 gccgctgcct tcgttaatat cctttgaata agccaacttt gaatcacaag acgcatacca 361 aacGGCATTC CGGTACTGTT GGTAAAGCCA CCATGGAAAA GGTAGGTTCA ACCACTGATG 421 CCTAGGCACA CCGAAACGAC TAACCCTAAT TCTTATCCTT TACTTCAGGC CAAAGCTTAA 481 Gcatggctag caaaggagaa gaactcttca ctggagttgt cccaattctt gttgaattag 541 atggtgatgt taacggccac aagttctctg tcagtggaga gggtgaaggt gatgcaacat 601 acggaaaact taccctgaag ttcatctgca ctactggcaa actgcctgtt ccctggccaa 661 cactagtcac tactctgtgc tatggtgttc aatgcttttc aagatacccg gatcatatga 721 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa aggaccatct 781 tcttcaaaga tgacggcaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc 841 ttgttaatag aatcgagtta aaaggtattg acttcaagga agatggcaac attctgggac 901 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga 961 atggaatcaa agCgaacttc aagacccgcc acaacattga agatggaagc gttcaactag 1021 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc 1081 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg 1141 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctgtacaact 1201 gatCTAGAGC ATGCACCGGT GATATCgcgg ccgcattagg caccccaggc tttacacttt 1261 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag 1321 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat 1381 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga 1441 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt 1501 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg 1561 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc 1621 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt 1681 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta 1741 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt 1801 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat 1861 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt 1921 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc 1981 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt 2041 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa 2101 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt 2161 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa 2221 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc 2281 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca 2341 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 2401 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 2461 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 2521 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 2581 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 2641 aatgtcaggc tcccttatac acagccagtc tgcagGATAT CGTCGACGAA TTCGGCCGGc 2701 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc 2761 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta 2821 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg 2881 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata 2941 aggatccgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg 3001 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag 3061 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 3121 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3181 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3241 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3301 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3361 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3421 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 3481 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3541 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 3601 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 3661 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 3721 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 3781 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 3841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 3901 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 3961 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 4021 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 4081 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 4141 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 4201 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 4261 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 4321 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 4381 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 4441 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 4501 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 4561 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 4621 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 4681 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 4741 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 4801 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 4861 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 4921 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 4981 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg 5041 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 5101 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 5161 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 5221 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 5281 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 5341 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 5401 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 5461 ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 5521 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact 5581 tggagcggcc gc //