LOCUS       pGL3\Promoter\sg        4080 bp    DNA     circular     12-NOV-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|498754724|
COMMENT     VNTDBDATE|498754762|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGL3 Promoter sgGFP|
COMMENT     VNTAUTHORNAME|Jenuwein|
FEATURES             Location/Qualifiers
     rep_origin      4986..5441
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     misc_marker     complement(3994..4854)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     rep_origin      3232..3232
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     primer_bind     2975..2994
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     polyA_signal    2688..2909
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_feature    2920..2924
                     /note="BamHI"
     misc_feature    2925..2929
                     /note="SalI"
     gene            1313..1972
                     /note="CamR"
     PCR_primer      1205..1226
                     /dnas_title="GCGGCCGCATTAGGCACCCCAG"
                     /pair=""
                     /primer="GCGGCCGCATTAGGCACCCCAG"
                     /current=0
     misc_feature    2314..2619
                     /note="ccdB survival"
     PCR_primer      complement(2631..2653)
                     /dnas_title="CTGCAGACTGGCTGTGTATAAGG"
                     /pair=""
                     /primer="CTGCAGACTGGCTGTGTATAAGG"
                     /current=0
     primer_bind     103..122
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
     polyA_signal    1..154
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    162..166
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     misc_feature    461..1180
                     /note="sgGFP"
     PCR_primer      403..422
                     /dnas_title="GGCACACCGAAACGACTAAC"
                     /pair="MHCI-control-03_fw"
                     /primer="GGCACACCGAAACGACTAAC"
                     /current=0
     PCR_primer      complement(575..598)
                     /dnas_title="CTTCAGGGTAAGTTTTCCGTATGT"
                     /pair="sgGFP_5'-02-rev"
                     /primer="CTTCAGGGTAAGTTTTCCGTATGT"
                     /current=0
     PCR_primer      complement(522..546)
                     /dnas_title="GAGAACTTGTGGCCGTTAACATCAC"
                     /pair="sgGFP-rev-seq"
                     /primer="GAGAACTTGTGGCCGTTAACATCAC"
                     /current=0
     misc_feature    449..456
                     /note="insert"
     PCR_primer      434..474
                     /dnas_title="TCCTTTACTTCAGGCCAAAGCTTCATGGCTAGCAAAGG"
                     /pair="QCII-mhc-HindIII-GFP_fw"
                     /primer="TCCTTTACTTCAGGCCAAAGCTTCATGGCTAGCAAAGG"
                     /current=0
     PCR_primer      complement(434..474)
                     /dnas_title="CCTTTGCTAGCCATGAAGCTTTGGCCTGAAGTAAAGGA"
                     /pair="QCII-mhc-HindIII-GFP_rev"
                     /primer="CCTTTGCTAGCCATGAAGCTTTGGCCTGAAGTAAAGGA"
                     /current=0
     misc_feature    451..456
                     /note="HindIII"
     misc_feature    454..459
                     /note="AflII"
     PCR_primer      complement(2631..2692)
                     /dnas_title="GTCTGCTCGAAGCGGCCGGCCGAATTCGTCGACGATATCCTGCAG
                     ACTGGCTGTGTATAAGG"
                     /pair="clone-IF-FseI-rev"
                     /primer="GTCTGCTCGAAGCGGCCGGCCGAATTCGTCGACGATATCCTGCAGACTG
                     GCTGTGTATAAGG"
                     /current=0
     PCR_primer      1166..1226
                     /dnas_title="GAACTGTACAACTGATCTAGAGCATGCACCGGTGATATCGCGGCC
                     GCATTAGGCACCCCAG"
                     /pair="clone-IF-Xba-fw"
                     /primer="GAACTGTACAACTGATCTAGAGCATGCACCGGTGATATCGCGGCCGCAT
                     TAGGCACCCCAG"
                     /current=0
     PCR_primer      1187..1201
                     /dnas_title="GCATGCACCGGTGAT"
                     /pair="PE_read_1_IF-EcoRV-09-fw"
                     /primer="GCATGCACCGGTGAT"
                     /current=0
     PCR_primer      1183..1197
                     /dnas_title="TAGAGCATGCACCGG"
                     /pair="PE_read_1_IF-AgeI-10-fw"
                     /primer="TAGAGCATGCACCGG"
                     /current=0
     PCR_primer      1177..1191
                     /dnas_title="CTGATCTAGAGCATG"
                     /pair="PE_read_1_IF-SphI-11-fw"
                     /primer="CTGATCTAGAGCATG"
                     /current=0
     PCR_primer      complement(2653..2667)
                     /dnas_title="TCGTCGACGATATCC"
                     /pair="PE_Read_2_IF-PstI-12-rev"
                     /primer="TCGTCGACGATATCC"
                     /current=0
     PCR_primer      complement(2657..2671)
                     /dnas_title="GAATTCGTCGACGAT"
                     /pair="PE_Read_2_IF-EcoRV-13-rev"
                     /primer="GAATTCGTCGACGAT"
                     /current=0
     PCR_primer      complement(2661..2675)
                     /dnas_title="GGCCGAATTCGTCGA"
                     /pair="PE_Read_2_IF-SalI-14-rev"
                     /primer="GGCCGAATTCGTCGA"
                     /current=1
     PCR_primer      177..193
                     /dnas_title="CGCGTGCTAGCCCGGGCTCGAGATCTGCGATC"
                     /pair=""
                     /primer="CGCGTGCTAGCCCGGGCTCGAGATCTGCGATC"
                     /current=0
     PCR_primer      193..193
                     /dnas_title="CTCGAGATCTGCGATCTGCATCTC"
                     /pair=""
                     /primer="CTCGAGATCTGCGATCTGCATCTC"
                     /current=0
     PCR_primer      182..192
                     /dnas_title="GCTAGCCCGGG"
                     /pair=""
                     /primer="GCTAGCCCGGG"
                     /current=0
     PCR_primer      185..193
                     /dnas_title="AGCCCGGGCTCGAGATCTGCGATC"
                     /pair="sgGFP rv seq"
                     /primer="AGCCCGGGCTCGAGATCTGCGATC"
                     /current=0
     misc_feature    203..335
                     /note="hsp70 core promotor"
     misc_feature    167..193
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     misc_feature    377..447
                     /note="mhc 16 intron"
     source          1..5571
                     /dnas_title="pGL3 Promoter sgGFP"
ORIGIN      
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcttacgcg
      181 tgctagcccg ggcTCGAgat ctgagcgccg gagtataaat agaggcgctt cgtctacgga
      241 gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata
      301 aacaagcgca gctgaacaag ctaaacaatc tgcagaagct tggcattccg gtaCTGTTGG
      361 TAAAGCCACC ATGGAAAAGG TAGGTTCAAC CACTGATGCC TAGGCACACC GAAACGACTA
      421 ACCCTAATTC TTATCCTTTA CTTCAGGCCA AAGCTTAAGc atggctagca aaggagaaga
      481 actcttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta acggccacaa
      541 gttctctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta ccctgaagtt
      601 catctgcact actggcaaac tgcctgttcc ctggccaaca ctagtcacta ctctgtgcta
      661 tggtgttcaa tgcttttcaa gatacccgga tcatatgaaa cggcatgact ttttcaagag
      721 tgccatgccc gaaggttatg tacaggaaag gaccatcttc ttcaaagatg acggcaacta
      781 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      841 aggtattgac ttcaaggaag atggcaacat tctgggacac aaattggaat acaactataa
      901 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag Cgaacttcaa
      961 gacccgccac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1021 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1081 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1141 tgctgggatt acacatggca tggatgaact gtacaactga tCTAGAGCAT GCACCGGTGA
     1201 TATCgcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     1261 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     1321 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1381 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1441 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1501 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     1561 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     1621 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1681 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1741 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1801 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1861 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     1921 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
     1981 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     2041 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     2101 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     2161 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     2221 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     2281 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     2341 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     2401 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     2461 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     2521 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     2581 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     2641 agccagtctg cagGATATCG TCGACGAATT CGGCCGGccg cttcgagcag acatgataag
     2701 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2761 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2821 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2881 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatctcgacc gatgcccttg
     2941 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     3001 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     3061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4981 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5041 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5101 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5161 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5221 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5281 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5341 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5401 atttaacgcg aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct
     5461 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct
     5521 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg c
//

