LOCUS       pGL3\Promoter\sg        4080 bp    DNA     circular     12-NOV-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|498754724|
COMMENT     VNTDBDATE|498754762|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGL3 Promoter sgGFP|
COMMENT     VNTAUTHORNAME|Jenuwein|
FEATURES             Location/Qualifiers
     primer_bind     3018..3037
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
     polyA_signal    2916..3069
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     rep_origin      2330..2785
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     misc_marker     complement(1338..2198)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     gene            4214..5866
                     /note="luc+ firefly"
     primer_bind     319..338
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     polyA_signal    30..251
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_feature    4016..4170
                     /note="DSCP Drosophila synthetic core promoter"
     misc_feature    4073..4078
                     /note="Inr"
     misc_feature    4044..4050
                     /note="TATA"
     misc_feature    4102..4107
                     /note="DPE"
     intron          3082..3943
                     /note="Zfh1 enhancer"
     rep_origin      576..576
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      564..1183
                     /note="pBR322 ori"
     source          1..5868
                     /dnas_title="pGL3 Promoter sgGFP"
ORIGIN      
        1 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
       61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
      241 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
      301 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
      361 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
      421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     2341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     2761 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa
     2821 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt
     2881 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac
     2941 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     3001 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     3061 catttctcta tcgataggta cCTTAAAAGT GCCAGTGCTT GTGGCGTGCA CGGAGAAAAA
     3121 AACCTTGAGG TCATATTTGA AAAAAAAAAA AAAAAACTAA ATTCCAAGTG AGTAGTTTAC
     3181 TCTTAGTCAC TATCGAGTGT GTGCTGTTTG TTTTTCGAAA TATGCATATC TTAGCTACTT
     3241 GCGTTGCTGT TTTTTTTTTT TTTTTTTTTT TTTTGACGAT TTGTCAACAG GAAATATGCA
     3301 TAAGGTTGGC TCGGGATCCG GATCGGATGG CATGGGGTTT GATTGGATGC CGTACTTGGC
     3361 ACCGCACTGC CGTTATCGGT TGGCTCATTT CCCGTTAGCC GCGAACAAAG CCCACGCATT
     3421 GTGCTACGAC CGCCGTGATG TGTTCAAAGA TCAAGTGCCC CCAGAACCAT CTCCTGCCAT
     3481 CCCTGCCATC CCTGCCCGCC TGCATCCATC GCGGGCATTC AGATTCCGAC TGCGCATGAG
     3541 CAGCGGTCTC CAACACGTAA GCAAGTGGAA GGCGAGGGAT AAGAACAAGT AACTTCTCTC
     3601 TTATCGGACG CTTCCAGGAA TTTCGATCCT CGCACCGCAT TCCGCAGGTT TAGTCACTGG
     3661 ATTCCACGGC TTTTCGAGCT GATTGTCCCA AGAAACATTA TTTTGGACAT GTGACTCACT
     3721 GACTCAAAGC TATTATCACT TCTCGGACAT ATGCGTGTAT ATAAAATATT TTTAAACATT
     3781 ATTAGGTATA CTATAAACTC TGCCTCATTC ATGCCTACAG AACTTCAGCC TTGACATTCT
     3841 ATATATAGAA ACCCTTACTA CGTCTTTACT CTTTAAATAG TTCTTTGGAT ATTATATTAG
     3901 CACATTGATG TGCTTTTTTT TTGGATGGTT GTGTCCAACT CACggtaccg agctcttaCG
     3961 CGTCCATGCA TGCTATGGGA ATTCCCATCA GACGTCTGGC TCGAGATCTG CGATCgagct
     4021 cgcccgggga tcgagcgcag cggtataaaa gggcgcgggg tggctgagag catcagttgt
     4081 gaatgaatgt tcgagccgag cagacgtgcc gctgccttcg ttaatatcct ttgaataagc
     4141 caactttgaa tcacaagacg cataccaaac CCTGCAggAa gcttggcatt ccggtactgt
     4201 tggtaaagcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     4261 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
     4321 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
     4381 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
     4441 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     4501 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     4561 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
     4621 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
     4681 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     4741 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
     4801 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
     4861 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     4921 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     4981 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag
     5041 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc
     5101 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg
     5161 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat
     5221 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     5281 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     5341 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat
     5401 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     5461 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga
     5521 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc
     5581 catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga
     5641 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     5701 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     5761 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     5821 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaatt
//

