LOCUS       pCAP003_pGL3_IF_        4939 bp    DNA     linear       17-JAN-2017
FEATURES             Location/Qualifiers
     rep_origin      1..456
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     PCR_primer      689..708
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
                     /current=0
     polyA_signal    587..740
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    2324..2394
                     /note="MHC intron 16"
     misc_feature    2327..2393
                     /note="intron spiced out"
     polyA_signal    2624..2845
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_marker     complement(3948..4808)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     gene            918..1577
                     /note="CamR"
     enhancer        789..803
                     /note="homology arm IF"
     misc_feature    1919..2224
                     /note="ccdB survival"
     enhancer        2266..2280
                     /note="homology arm IF"
     misc_feature    2405..2593
                     /note="truncated sgGFP"
     primer_bind     2927..2948
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     rep_origin      3186..3186
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      3174..3793
                     /note="pBR322 rep_ori"
     source          1..4939
                     /dnas_title="pCAP003_pGL3_IF_mhcI_tGFP_CapSeq_cleaned"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt accgagctct tacgcgtgct agcccgggcT
      781 CGAGATCTTA GAGCATGCAC CGGTGATATC cggccgcatt aggcacccca ggctttacac
      841 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
      901 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
      961 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1021 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     1081 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     1141 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     1201 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     1261 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     1321 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     1381 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     1441 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     1501 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     1561 ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt
     1621 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     1681 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     1741 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     1801 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     1861 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     1921 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     1981 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     2041 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     2101 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     2161 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     2221 ataaatgtca ggctccctta tacacagcca gtctgcagGA TATCGTCGAC GAATTCGGCC
     2281 CCTGCAGGGG CATTCCGGTA CTGTTGGTAA AGCCACCATG GAAAAGGTAG GTTCAACCAC
     2341 TGATGCCTAG GCACACCGAA ACGACTAACC CTAATTCTTA TCCTTTACTT CAGGCCACTT
     2401 AAGcatggct agcaaaggag aagaactctt cactggagtt gtcccaattc ttgttgaatt
     2461 agatggtgat gttaacggcc acaagttctc tgtcagtgga gagggtgaag gtgatgcaac
     2521 atacggaaaa cttaccctga agttcatctg cactactggc aaactgcctg ttccctggcc
     2581 aacactagtc tgatctagag tcggggcGGC CGGccgcttc gagcagacat gataagatac
     2641 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2701 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     2761 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     2821 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgaccgatgc cctCAAGCTT
     2881 tgagagGGGC Ccccttcaac ccagtcagct ccGACGTCtt ggCgccCCcg cggggcatga
     2941 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     3001 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3061 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3601 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3781 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3901 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3961 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4021 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4081 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4141 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4201 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4261 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4321 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4381 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4441 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4501 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4561 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4621 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4681 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4741 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4801 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4861 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4921 ccccgaaaag tgccacctg
//

