LOCUS pCAP005_pGL3_enh 5509 bp DNA linear 17-JAN-2017 FEATURES Location/Qualifiers rep_origin 1..456 /dnas_title="f1 origin" /vntifkey="33" /label=f1 origin PCR_primer 689..708 /dnas_title="RVprimer3 binding region" /vntifkey="28" /label=RVprimer3 binding region /current=0 polyA_signal 587..740 /dnas_title="Synthetic (upstream) poly (A)signal" /vntifkey="25" /label=Synthetic (upstream) poly (A)signal misc_feature 2894..2964 /note="MHC intron 16" misc_feature 2897..2963 /note="intron spiced out" polyA_signal 3194..3415 /dnas_title="SV40 late poly (A) signal" /vntifkey="25" /label=SV40 late poly (A) signal misc_marker complement(4518..5378) /dnas_title="Beta-lactamase gene (Ampr)" /vntifkey="22" /label=Beta-lactamase gene (Ampr) gene 1488..2147 /note="CamR" enhancer 1359..1373 /note="homology arm IF" misc_feature 2489..2794 /note="ccdB survival" enhancer 2836..2850 /note="homology arm IF" misc_feature 2975..3163 /note="truncated sgGFP" primer_bind 3497..3518 /dnas_title="RVprimer4 binding site" /vntifkey="28" /label=RVprimer4 binding site rep_origin 3756..3756 /dnas_title="ColE1-derived plasmid replication origin" /vntifkey="33" /label=ColE1-derived plasmid replication origin rep_origin 3744..4363 /note="pBR322 rep_ori" enhancer 754..1352 /note="ham enhancer" source 1..5509 /dnas_title="pCAP005_pGL3_enh9-1-ham_IF_CapSeq_cleaned" ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca 481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat 541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt 601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct 661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc 721 aggtgccaga acatttctct atcgataggt accTTGCGCA CTGTGCTGCG ACTTGCAGAT 781 CGATTTTCAG ACGCTATCCT AAAGATCCAT GCGGGTGCAT AAGAAAAGAT ACAAATACAG 841 ATACAACTAC AAATACAGGG AATCGGACTG ATCTGAATGG CAGGGCAGAC AAACCGCAGT 901 TTGAGATGTT AAGTTGTTTG TTTGTTGTGG GCACTTCGGA ATCGACCCTG TTTGGATTGC 961 TGATAAATGT CTAAAAATAC GCAAGGCAAC CACAACATGA AAGATACAAT GTGTGCCGAG 1021 TTGGAAGGGT TCCACGGAGT CTGCTCTTCG TCTGGCTTTA TCTCGTTCGG AATTTTCTGA 1081 ATTTTATCGA CAACATAAAG CAATTGAATT GCAACATAAA TCAGAGACCA CAACATGTGT 1141 TGAGCACTCG ATGATAGCGG TATGATGTAT TTATATTCAA AGTAATATAT CTGAGTCCGC 1201 CATTTTCCCC ACAATTTCCC CATCCCCTTG CTTTCAACCG GCTTTTTAAT CCCCAGCCCC 1261 CATTTGGCGG CCATAGCCAA GCGTAAAGAG ATGTAAAGAC ATGCTCGAGA GTGCCACTCA 1321 ATGTGGCAAG CCATGGAGAA TGGGAAATGG GGAGATCTTA GAGCATGCAC CGGTGATATC 1381 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga 1441 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa 1501 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat 1561 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt 1621 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc 1681 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat 1741 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc 1801 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg 1861 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg 1921 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca 1981 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga 2041 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc 2101 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg 2161 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa 2221 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat 2281 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc 2341 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg 2401 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct 2461 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga 2521 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg 2581 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta 2641 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt 2701 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa 2761 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca 2821 gtctgcagGA TATCGTCGAC GAATTCGGCC CCTGCAGGGG CATTCCGGTA CTGTTGGTAA 2881 AGCCACCATG GAAAAGGTAG GTTCAACCAC TGATGCCTAG GCACACCGAA ACGACTAACC 2941 CTAATTCTTA TCCTTTACTT CAGGCCACTT AAGcatggct agcaaaggag aagaactctt 3001 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaacggcc acaagttctc 3061 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctga agttcatctg 3121 cactactggc aaactgcctg ttccctggcc aacactagtc tgatctagag tcggggcGGC 3181 CGGccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga 3241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 3301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 3361 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc 3421 gataaggatc cgaccgatgc cctCAAGCTT tgagagGGGC Ccccttcaac ccagtcagct 3481 ccGACGTCtt ggCgccCCcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 3541 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac 3601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 4201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4801 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 4861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 4921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 4981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 5041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 5101 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 5161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 5221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 5281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 5341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 5401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 5461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg //