LOCUS       pSTAP-seq_fly-nc        5793 bp    DNA     linear       17-JAN-2017
FEATURES             Location/Qualifiers
     rep_origin      1..456
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     PCR_primer      689..708
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
                     /current=0
     polyA_signal    587..740
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    3178..3248
                     /note="MHC intron 16"
     misc_feature    3181..3247
                     /note="intron spiced out"
     polyA_signal    3478..3699
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_marker     complement(4802..5662)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     gene            1772..2431
                     /note="CamR"
     enhancer        1643..1657
                     /note="homology arm IF"
     misc_feature    2773..3078
                     /note="ccdB survival"
     enhancer        3120..3134
                     /note="homology arm IF"
     misc_feature    3259..3447
                     /note="truncated sgGFP"
     primer_bind     3781..3802
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     rep_origin      4040..4040
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      4028..4647
                     /note="pBR322 rep_ori"
     enhancer        754..1636
                     /note="ncm enhancer"
     source          1..5793
                     /dnas_title="pSTAP-seq_fly-ncm"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt accGCTGGAC AGGTGTTTCA ACTTTTTTCG
      781 CTTCATTTAA TTTCTCGCTT TCCGCCTCCG CAGCTGCTAC TGCTTCTTCT TGCTGCTTTT
      841 CCACGCCAGC AGAATGGGAA ATGTGCTGGC GCTCCGAACG AGGCTCAGCC GCAGGAGTTT
      901 TCTTCTGCTC AGTGGGCTGC TCATCCTGTC CAGCATCCCT CTCCTTGGCA CGCTTCTCCC
      961 CCCTATCCGT ATCGTCAGTT TTCTTGGCAT CTTTGGCGGA TTCCTCCTGC TTTGCTTCCT
     1021 CTTCCTCGCG CCCGCTGCTT TCGGAGGACG AGGACTCGCT CCTGGCGTCG CTGTCGCTGC
     1081 CACTGGAAGT GTCGCTGCTG CTGGAATTGC TGCTCGAATC GCTCTCGGCA TCGCTTTCCC
     1141 CCATCGCGCG TTTATTTCGA GAAATTTTCA GGGAAAACAA CGATGAAAAT TATTGGGCAG
     1201 CAAAAAACAC AACGCGGCGA TAGAATTAGA GATGGGACAC CAGTGGCGAC GGTATCGATA
     1261 TACACATGTG TTTGACTGCT AAATCGTATC GAAATTGTAG TTTCGATAGT CAGCTATAGA
     1321 AATGCTAAAT GTGTTTTTTA AATTGAACTG AAAATTTAAA TTCAAGTTCC ACATTAGAAA
     1381 AAATGATATT TTAAAATTAA TTTTATAATA TGGCACTATC GTAAATTTTA TAGCACAGCG
     1441 AACCTCAATA TTTAAGGGCA ACGATTTTTT TACGACTAAA ACAGTGTAAT ATATTTGTTA
     1501 TAACATATTT GGTTCATTGC ACTGCAGAAA TTTCGAATAT CAGCTCGAAA ATGGAACAAG
     1561 GATATGGAAC AAGAACATTA AATTTGAAAC GAAACTTTTT TTTTTTAGCG ATTTTTGGGC
     1621 TCCAAAACGG GCAATCAGAT CTTAGAGCAT GCACCGGTGA TATCcggccg cattaggcac
     1681 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
     1741 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     1801 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     1861 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     1921 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     1981 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     2041 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     2101 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     2161 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     2221 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     2281 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     2341 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     2401 gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag
     2461 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
     2521 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
     2581 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
     2641 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
     2701 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
     2761 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     2821 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
     2881 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
     2941 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     3001 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     3061 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc agGATATCGT
     3121 CGACGAATTC GGCCCCTGCA GGGGCATTCC GGTACTGTTG GTAAAGCCAC CATGGAAAAG
     3181 GTAGGTTCAA CCACTGATGC CTAGGCACAC CGAAACGACT AACCCTAATT CTTATCCTTT
     3241 ACTTCAGGCC ACTTAAGcat ggctagcaaa ggagaagaac tcttcactgg agttgtccca
     3301 attcttgttg aattagatgg tgatgttaac ggccacaagt tctctgtcag tggagagggt
     3361 gaaggtgatg caacatacgg aaaacttacc ctgaagttca tctgcactac tggcaaactg
     3421 cctgttccct ggccaacact agtctgatct agagtcgggg cGGCCGGccg cttcgagcag
     3481 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     3541 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3601 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     3661 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgaccg
     3721 atgccctCAA GCTTtgagag GGGCCccctt caacccagtc agctccGACG TCttggCgcc
     3781 CCcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     3841 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3901 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3961 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4021 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4081 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4141 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4201 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4261 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4321 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4381 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4441 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4501 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4621 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4681 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4741 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4801 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4861 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4921 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4981 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5041 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5101 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5161 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5221 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5281 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5341 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5401 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5461 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5521 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5581 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5641 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5701 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5761 ttccgcgcac atttccccga aaagtgccac ctg
//

