LOCUS pSTAP-seq_fly-nc 5793 bp DNA linear 17-JAN-2017 FEATURES Location/Qualifiers rep_origin 1..456 /dnas_title="f1 origin" /vntifkey="33" /label=f1 origin PCR_primer 689..708 /dnas_title="RVprimer3 binding region" /vntifkey="28" /label=RVprimer3 binding region /current=0 polyA_signal 587..740 /dnas_title="Synthetic (upstream) poly (A)signal" /vntifkey="25" /label=Synthetic (upstream) poly (A)signal misc_feature 3178..3248 /note="MHC intron 16" misc_feature 3181..3247 /note="intron spiced out" polyA_signal 3478..3699 /dnas_title="SV40 late poly (A) signal" /vntifkey="25" /label=SV40 late poly (A) signal misc_marker complement(4802..5662) /dnas_title="Beta-lactamase gene (Ampr)" /vntifkey="22" /label=Beta-lactamase gene (Ampr) gene 1772..2431 /note="CamR" enhancer 1643..1657 /note="homology arm IF" misc_feature 2773..3078 /note="ccdB survival" enhancer 3120..3134 /note="homology arm IF" misc_feature 3259..3447 /note="truncated sgGFP" primer_bind 3781..3802 /dnas_title="RVprimer4 binding site" /vntifkey="28" /label=RVprimer4 binding site rep_origin 4040..4040 /dnas_title="ColE1-derived plasmid replication origin" /vntifkey="33" /label=ColE1-derived plasmid replication origin rep_origin 4028..4647 /note="pBR322 rep_ori" enhancer 754..1636 /note="ncm enhancer" source 1..5793 /dnas_title="pSTAP-seq_fly-ncm" ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca 481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat 541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt 601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct 661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc 721 aggtgccaga acatttctct atcgataggt accGCTGGAC AGGTGTTTCA ACTTTTTTCG 781 CTTCATTTAA TTTCTCGCTT TCCGCCTCCG CAGCTGCTAC TGCTTCTTCT TGCTGCTTTT 841 CCACGCCAGC AGAATGGGAA ATGTGCTGGC GCTCCGAACG AGGCTCAGCC GCAGGAGTTT 901 TCTTCTGCTC AGTGGGCTGC TCATCCTGTC CAGCATCCCT CTCCTTGGCA CGCTTCTCCC 961 CCCTATCCGT ATCGTCAGTT TTCTTGGCAT CTTTGGCGGA TTCCTCCTGC TTTGCTTCCT 1021 CTTCCTCGCG CCCGCTGCTT TCGGAGGACG AGGACTCGCT CCTGGCGTCG CTGTCGCTGC 1081 CACTGGAAGT GTCGCTGCTG CTGGAATTGC TGCTCGAATC GCTCTCGGCA TCGCTTTCCC 1141 CCATCGCGCG TTTATTTCGA GAAATTTTCA GGGAAAACAA CGATGAAAAT TATTGGGCAG 1201 CAAAAAACAC AACGCGGCGA TAGAATTAGA GATGGGACAC CAGTGGCGAC GGTATCGATA 1261 TACACATGTG TTTGACTGCT AAATCGTATC GAAATTGTAG TTTCGATAGT CAGCTATAGA 1321 AATGCTAAAT GTGTTTTTTA AATTGAACTG AAAATTTAAA TTCAAGTTCC ACATTAGAAA 1381 AAATGATATT TTAAAATTAA TTTTATAATA TGGCACTATC GTAAATTTTA TAGCACAGCG 1441 AACCTCAATA TTTAAGGGCA ACGATTTTTT TACGACTAAA ACAGTGTAAT ATATTTGTTA 1501 TAACATATTT GGTTCATTGC ACTGCAGAAA TTTCGAATAT CAGCTCGAAA ATGGAACAAG 1561 GATATGGAAC AAGAACATTA AATTTGAAAC GAAACTTTTT TTTTTTAGCG ATTTTTGGGC 1621 TCCAAAACGG GCAATCAGAT CTTAGAGCAT GCACCGGTGA TATCcggccg cattaggcac 1681 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc 1741 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac 1801 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca 1861 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa 1921 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca 1981 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc 2041 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca 2101 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa 2161 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg 2221 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt 2281 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 2341 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 2401 gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag 2461 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta 2521 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac 2581 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca 2641 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg 2701 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca 2761 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg 2821 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg 2881 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc 2941 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc 3001 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg 3061 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc agGATATCGT 3121 CGACGAATTC GGCCCCTGCA GGGGCATTCC GGTACTGTTG GTAAAGCCAC CATGGAAAAG 3181 GTAGGTTCAA CCACTGATGC CTAGGCACAC CGAAACGACT AACCCTAATT CTTATCCTTT 3241 ACTTCAGGCC ACTTAAGcat ggctagcaaa ggagaagaac tcttcactgg agttgtccca 3301 attcttgttg aattagatgg tgatgttaac ggccacaagt tctctgtcag tggagagggt 3361 gaaggtgatg caacatacgg aaaacttacc ctgaagttca tctgcactac tggcaaactg 3421 cctgttccct ggccaacact agtctgatct agagtcgggg cGGCCGGccg cttcgagcag 3481 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 3541 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 3601 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 3661 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgaccg 3721 atgccctCAA GCTTtgagag GGGCCccctt caacccagtc agctccGACG TCttggCgcc 3781 CCcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt 3841 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3901 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3961 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4021 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4081 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4141 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4201 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4261 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4321 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4381 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4441 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 4501 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4621 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4681 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 4741 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 4801 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4861 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4921 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4981 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 5041 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 5101 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 5161 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 5221 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 5281 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 5341 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5401 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5461 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 5521 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 5581 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 5641 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 5701 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 5761 ttccgcgcac atttccccga aaagtgccac ctg //