LOCUS       pSTAP-seq_fly-ss        5509 bp    DNA     linear       17-JAN-2017
FEATURES             Location/Qualifiers
     rep_origin      1..456
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     PCR_primer      689..708
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
                     /current=0
     polyA_signal    587..740
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    2894..2964
                     /note="MHC intron 16"
     misc_feature    2897..2963
                     /note="intron spiced out"
     polyA_signal    3194..3415
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_marker     complement(4518..5378)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     gene            1488..2147
                     /note="CamR"
     enhancer        1359..1373
                     /note="homology arm IF"
     misc_feature    2489..2794
                     /note="ccdB survival"
     enhancer        2836..2850
                     /note="homology arm IF"
     misc_feature    2975..3163
                     /note="truncated sgGFP"
     primer_bind     3497..3518
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     rep_origin      3756..3756
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      3744..4363
                     /note="pBR322 rep_ori"
     enhancer        754..1352
                     /note="ssp3 enhancer"
     source          1..5509
                     /dnas_title="pSTAP-seq_fly-ssp3"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt accTTTGTTG CTCCTATTTC AGACGTCGCT
      781 TCTTTGTTGT TTTTATCGCA GAATTTTGCA ACGCCAGTGG ACTTGGTAGC ATTTTCCAAA
      841 GGCGTAACGA GTCTAAAGAA ACCGCGATGT GTTCAATCAA TGCCTGAAAG ATTCCCCTTC
      901 ACTATTCCAG TTGTCTGCTG CTTGTCTTGT GTCGGTTAGA GAGAAATTAG AATTTTTGAT
      961 TTCTGCCAAA CTCAGCCGAG CAGAGCCAGC TGATTTAAGC GTTGGGCTGC AATTGTGCAA
     1021 TATCTGTCAT TGGGTAATCG ATAGAAGCTA AACTATCGAT ACGAGCGCGC ACTTAATCGA
     1081 CATTATAAGT AAATTCAATT GTAAGAGCAA CTTTTTGAGT ATTCAACAAA AAAAACAGAA
     1141 AAAATACCAA ACACAATTAT TTCACATTAA CGCTTACAAA AACTTTCTAT AAAAATGCAC
     1201 AATAATTCTT TTGTTTACAG GAGAAAAGTA AAACAGCGAA AGAGAAAAAA AGAACTCAAA
     1261 TTTGAAATTT TTGGATTACA AAATTGCCTT CAAAATTGGC CCTTAACCGC AAAGCTCCAT
     1321 CCGAAAGCTC ACCGCGGGCG AGCGAGACAA CCAGATCTTA GAGCATGCAC CGGTGATATC
     1381 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
     1441 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     1501 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     1561 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     1621 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1681 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     1741 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1801 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1861 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1921 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1981 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     2041 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     2101 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg
     2161 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     2221 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     2281 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     2341 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     2401 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     2461 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     2521 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     2581 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     2641 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     2701 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     2761 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     2821 gtctgcagGA TATCGTCGAC GAATTCGGCC CCTGCAGGGG CATTCCGGTA CTGTTGGTAA
     2881 AGCCACCATG GAAAAGGTAG GTTCAACCAC TGATGCCTAG GCACACCGAA ACGACTAACC
     2941 CTAATTCTTA TCCTTTACTT CAGGCCACTT AAGcatggct agcaaaggag aagaactctt
     3001 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaacggcc acaagttctc
     3061 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctga agttcatctg
     3121 cactactggc aaactgcctg ttccctggcc aacactagtc tgatctagag tcggggcGGC
     3181 CGGccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3361 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     3421 gataaggatc cgaccgatgc cctCAAGCTT tgagagGGGC Ccccttcaac ccagtcagct
     3481 ccGACGTCtt ggCgccCCcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     3541 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     3601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4801 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5101 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//

