LOCUS       pSTAP-seq_fly-tj        5766 bp    DNA     linear       17-JAN-2017
FEATURES             Location/Qualifiers
     rep_origin      1..456
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     PCR_primer      689..708
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
                     /current=0
     polyA_signal    587..740
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    3151..3221
                     /note="MHC intron 16"
     misc_feature    3154..3220
                     /note="intron spiced out"
     polyA_signal    3451..3672
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_marker     complement(4775..5635)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     gene            1745..2404
                     /note="CamR"
     enhancer        1616..1630
                     /note="homology arm IF"
     misc_feature    2746..3051
                     /note="ccdB survival"
     enhancer        3093..3107
                     /note="homology arm IF"
     misc_feature    3232..3420
                     /note="truncated sgGFP"
     primer_bind     3754..3775
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     rep_origin      4013..4013
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      4001..4620
                     /note="pBR322 rep_ori"
     enhancer        754..1609
                     /note="tj enhancer"
     source          1..5766
                     /dnas_title="pSTAP-seq_fly-tj"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt accTTCAGTG CAACTGTAAA CATTGATACG
      781 GATTAAAAGA TTCATTGAAT GCTCATGAAC TGAGAGTTAC GTTCGTTTCT TTGGATGCTT
      841 GATAGTTTAA ACATTGAAGA TCGTAAAAAT ATAGAATGCT CAGTTTTTCA TAGTATTCAA
      901 ATTGTATTCT ATTTTGTCCC CATGCCACAT TCATATTCAT ATCTGGGTTT TAGAAACCAC
      961 CGAGTGGGTC TCAGCTTGCA TCATAGATTT TGGGTTTCAT CTCGCCGTTT TCCGAGAAGT
     1021 GGCATCTTCT GTGTGGTCTG TCGGCATGGA TAAATGGGCT TCCAGCGACG ACGACCCCCG
     1081 CGGGCAACAG TTTAAGAATC AAAATAAACA CTTCGATCCC GATCCCTCTG GACTTCGATA
     1141 AGTTATGTCA ACAAACTTCG GTCGCGTAAC GGCGCATGCA GGAGCTCTGG AGTAGTAGGA
     1201 GCTCCAGCTG AAGTGCAGCA TTGGGTTACA ATCGTCCACA TGGAGGAGAT CAGCATCAGC
     1261 CCCTAGGAGG GGGAGGGGGC GGTGTTGGAA CTTTGGAGCA GTCCAGCATT CAGTTGGGGC
     1321 GGCGTAATTC GGCAATAATC AGAGGGCCGT ATCTACATAT CTGCATTTTC AAACCGTAAT
     1381 AAATCCCTAA TAAAAATCGT TTCCTTTGGC CTGTTTATTT TCATTTCAAT TTCGATTCAC
     1441 TTTTGCGGTG TCGCCTCGCT ACGCTTTTTT CAGAATCTGA ATTTGTTATT TTTATTTTGA
     1501 TTATATGAGT TTCCTGCCCT ACCAAGAACC CAAATCTCTT AGGAGCTTAT GTAACCAGAC
     1561 GAAACCACTC GATCGAAGAG TTGCGCAAGT ATTTTTGATG AAATTCGGGA GATCTTAGAG
     1621 CATGCACCGG TGATATCcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     1681 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     1741 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     1801 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     1861 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     1921 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     1981 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     2041 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     2101 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     2161 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     2221 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     2281 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     2341 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     2401 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     2461 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     2521 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     2581 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     2641 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     2701 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     2761 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     2821 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     2881 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     2941 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     3001 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     3061 tcccttatac acagccagtc tgcagGATAT CGTCGACGAA TTCGGCCCCT GCAGGGGCAT
     3121 TCCGGTACTG TTGGTAAAGC CACCATGGAA AAGGTAGGTT CAACCACTGA TGCCTAGGCA
     3181 CACCGAAACG ACTAACCCTA ATTCTTATCC TTTACTTCAG GCCACTTAAG catggctagc
     3241 aaaggagaag aactcttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     3301 aacggccaca agttctctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     3361 accctgaagt tcatctgcac tactggcaaa ctgcctgttc cctggccaac actagtctga
     3421 tctagagtcg gggcGGCCGG ccgcttcgag cagacatgat aagatacatt gatgagtttg
     3481 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3541 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3601 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     3661 acaaatgtgg taaaatcgat aaggatccga ccgatgccct CAAGCTTtga gagGGGCCcc
     3721 cttcaaccca gtcagctccG ACGTCttggC gccCCcgcgg ggcatgacta tcgtcgccgc
     3781 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     3841 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3901 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3961 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4021 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4081 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4141 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4201 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4261 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4321 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4381 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4441 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4501 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4561 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4621 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4681 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4741 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4801 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4861 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4921 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4981 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5041 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5101 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5161 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5221 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5281 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5341 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5401 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5461 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5521 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5581 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5641 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5701 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5761 cacctg
//

