LOCUS       pCAP002_pGL3_zfh        4571 bp    DNA     linear       17-JAN-2017
FEATURES             Location/Qualifiers
     rep_origin      1..456
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     PCR_primer      689..708
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
                     /current=0
     polyA_signal    587..740
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    1956..2026
                     /note="MHC intron 16"
     misc_feature    1959..2025
                     /note="intron spiced out"
     polyA_signal    2256..2477
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_marker     complement(3580..4440)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     misc_feature    2037..2225
                     /note="truncated sgGFP"
     primer_bind     2559..2580
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     rep_origin      2818..2818
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      2806..3425
                     /note="pBR322 rep_ori"
     intron          753..1614
                     /note="Zfh1 enhancer"
     enhancer        1898..1912
                     /note="homology arm IF"
     PCR_primer      complement(1864..1897)
                     /note="illumina adaptor rv"
     PCR_primer      1671..1703
                     /note="illumina adapter fw"
     promoter        1704..1863
                     /note="dpse eve promoter"
     enhancer        1656..1670
                     /note="homology arm IF"
     source          1..4571
                     /dnas_title="pCAP002_pGL3_zfh1_dpse-eve_IF_CapSeq_cleaned"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt acCTTAAAAG TGCCAGTGCT TGTGGCGTGC
      781 ACGGAGAAAA AAACCTTGAG GTCATATTTG AAAAAAAAAA AAAAAAACTA AATTCCAAGT
      841 GAGTAGTTTA CTCTTAGTCA CTATCGAGTG TGTGCTGTTT GTTTTTCGAA ATATGCATAT
      901 CTTAGCTACT TGCGTTGCTG TTTTTTTTTT TTTTTTTTTT TTTTTGACGA TTTGTCAACA
      961 GGAAATATGC ATAAGGTTGG CTCGGGATCC GGATCGGATG GCATGGGGTT TGATTGGATG
     1021 CCGTACTTGG CACCGCACTG CCGTTATCGG TTGGCTCATT TCCCGTTAGC CGCGAACAAA
     1081 GCCCACGCAT TGTGCTACGA CCGCCGTGAT GTGTTCAAAG ATCAAGTGCC CCCAGAACCA
     1141 TCTCCTGCCA TCCCTGCCAT CCCTGCCCGC CTGCATCCAT CGCGGGCATT CAGATTCCGA
     1201 CTGCGCATGA GCAGCGGTCT CCAACACGTA AGCAAGTGGA AGGCGAGGGA TAAGAACAAG
     1261 TAACTTCTCT CTTATCGGAC GCTTCCAGGA ATTTCGATCC TCGCACCGCA TTCCGCAGGT
     1321 TTAGTCACTG GATTCCACGG CTTTTCGAGC TGATTGTCCC AAGAAACATT ATTTTGGACA
     1381 TGTGACTCAC TGACTCAAAG CTATTATCAC TTCTCGGACA TATGCGTGTA TATAAAATAT
     1441 TTTTAAACAT TATTAGGTAT ACTATAAACT CTGCCTCATT CATGCCTACA GAACTTCAGC
     1501 CTTGACATTC TATATATAGA AACCCTTACT ACGTCTTTAC TCTTTAAATA GTTCTTTGGA
     1561 TATTATATTA GCACATTGAT GTGCTTTTTT TTTGGATGGT TGTGTCCAAC TCACggtacc
     1621 gagctcttac gcgtgctagc ccgggcTCGA GATCTTAGAG CATGCACCGG ACACTCTTTC
     1681 CCTACACGAC GCTCTTCCGA TCTCAGGCAC GGCCGCAGAG GTATATAAGG GCCGCGGGGT
     1741 GGCCGAGAGA GGCACATTCG AACTGTAACC GTCGCACAGT CCAGACACAC TCGATCCGCT
     1801 CCGAAAAGAG AACCGCAGTT TCCAGTTCTT AGAGACAACG CCCTCACCAG CCCATCAACA
     1861 TCCAGATCGG AAGAGCACAC GTCTGAACTC CAGTCACTCG ACGAATTCGG CCCCTGCAGG
     1921 GGCATTCCGG TACTGTTGGT AAAGCCACCA TGGAAAAGGT AGGTTCAACC ACTGATGCCT
     1981 AGGCACACCG AAACGACTAA CCCTAATTCT TATCCTTTAC TTCAGGCCAC TTAAGcatgg
     2041 ctagcaaagg agaagaactc ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     2101 atgttaacgg ccacaagttc tctgtcagtg gagagggtga aggtgatgca acatacggaa
     2161 aacttaccct gaagttcatc tgcactactg gcaaactgcc tgttccctgg ccaacactag
     2221 tctgatctag agtcggggcG GCCGGccgct tcgagcagac atgataagat acattgatga
     2281 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2341 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2401 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2461 cctctacaaa tgtggtaaaa tcgataagga tccgaccgat gccctCAAGC TTtgagagGG
     2521 GCCcccttca acccagtcag ctccGACGTC ttggCgccCC cgcggggcat gactatcgtc
     2581 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     2641 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2701 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2761 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2821 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2881 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2941 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3001 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3061 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3121 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3181 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3241 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3301 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3361 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3421 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3481 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3541 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3601 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3661 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3721 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3781 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3841 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3901 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3961 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4021 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4081 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4141 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4201 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4261 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4321 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4381 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4441 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4501 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4561 agtgccacct g
//

