LOCUS       pSTAP-seq_fly-zf        4581 bp    DNA     linear       17-JAN-2017
FEATURES             Location/Qualifiers
     rep_origin      1..456
                     /dnas_title="f1 origin"
                     /vntifkey="33"
                     /label=f1 origin
     PCR_primer      689..708
                     /dnas_title="RVprimer3 binding region"
                     /vntifkey="28"
                     /label=RVprimer3 binding region
                     /current=0
     polyA_signal    587..740
                     /dnas_title="Synthetic (upstream) poly (A)signal"
                     /vntifkey="25"
                     /label=Synthetic (upstream) poly (A)signal
     misc_feature    1966..2036
                     /note="MHC intron 16"
     misc_feature    1969..2035
                     /note="intron spiced out"
     polyA_signal    2266..2487
                     /dnas_title="SV40 late poly (A) signal"
                     /vntifkey="25"
                     /label=SV40 late poly (A) signal
     misc_marker     complement(3590..4450)
                     /dnas_title="Beta-lactamase gene (Ampr)"
                     /vntifkey="22"
                     /label=Beta-lactamase gene (Ampr)
     misc_feature    2047..2235
                     /note="truncated sgGFP"
     primer_bind     2569..2590
                     /dnas_title="RVprimer4 binding site"
                     /vntifkey="28"
                     /label=RVprimer4 binding site
     rep_origin      2828..2828
                     /dnas_title="ColE1-derived plasmid replication origin"
                     /vntifkey="33"
                     /label=ColE1-derived plasmid replication origin
     rep_origin      2816..3435
                     /note="pBR322 rep_ori"
     intron          753..1614
                     /note="Zfh1 enhancer"
     enhancer        1908..1922
                     /note="homology arm IF"
     PCR_primer      complement(1874..1907)
                     /note="illumina adaptor rv"
     PCR_primer      1671..1703
                     /note="illumina adapter fw"
     enhancer        1656..1670
                     /note="homology arm IF"
     promoter        1704..1873
                     /note="dpse ush-1 promoter"
     source          1..4581
                     /dnas_title="pSTAP-seq_fly-zfh1_spikeIn-3"
ORIGIN      
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat
      541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt
      601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct
      661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc
      721 aggtgccaga acatttctct atcgataggt acCTTAAAAG TGCCAGTGCT TGTGGCGTGC
      781 ACGGAGAAAA AAACCTTGAG GTCATATTTG AAAAAAAAAA AAAAAAACTA AATTCCAAGT
      841 GAGTAGTTTA CTCTTAGTCA CTATCGAGTG TGTGCTGTTT GTTTTTCGAA ATATGCATAT
      901 CTTAGCTACT TGCGTTGCTG TTTTTTTTTT TTTTTTTTTT TTTTTGACGA TTTGTCAACA
      961 GGAAATATGC ATAAGGTTGG CTCGGGATCC GGATCGGATG GCATGGGGTT TGATTGGATG
     1021 CCGTACTTGG CACCGCACTG CCGTTATCGG TTGGCTCATT TCCCGTTAGC CGCGAACAAA
     1081 GCCCACGCAT TGTGCTACGA CCGCCGTGAT GTGTTCAAAG ATCAAGTGCC CCCAGAACCA
     1141 TCTCCTGCCA TCCCTGCCAT CCCTGCCCGC CTGCATCCAT CGCGGGCATT CAGATTCCGA
     1201 CTGCGCATGA GCAGCGGTCT CCAACACGTA AGCAAGTGGA AGGCGAGGGA TAAGAACAAG
     1261 TAACTTCTCT CTTATCGGAC GCTTCCAGGA ATTTCGATCC TCGCACCGCA TTCCGCAGGT
     1321 TTAGTCACTG GATTCCACGG CTTTTCGAGC TGATTGTCCC AAGAAACATT ATTTTGGACA
     1381 TGTGACTCAC TGACTCAAAG CTATTATCAC TTCTCGGACA TATGCGTGTA TATAAAATAT
     1441 TTTTAAACAT TATTAGGTAT ACTATAAACT CTGCCTCATT CATGCCTACA GAACTTCAGC
     1501 CTTGACATTC TATATATAGA AACCCTTACT ACGTCTTTAC TCTTTAAATA GTTCTTTGGA
     1561 TATTATATTA GCACATTGAT GTGCTTTTTT TTTGGATGGT TGTGTCCAAC TCACggtacc
     1621 gagctcttac gcgtgctagc ccgggcTCGA GATCTTAGAG CATGCACCGG ACACTCTTTC
     1681 CCTACACGAC GCTCTTCCGA TCTTTTGTGC GAGCTTTCGT CGATTTTGGA GCGAAGCAGC
     1741 AGCCCCAACG CGTTTGGCGA CGCCAACCTG CTTACAGTTT TAGTTGGGAG CTTGCACTGT
     1801 TCGGACGTTC AAAAGCCCAC AAGCCACGCC CCCACCGACA AGGCACTGGC AAAACACGCG
     1861 ACAAGGCAGC AACAGATCGG AAGAGCACAC GTCTGAACTC CAGTCACTCG ACGAATTCGG
     1921 CCCCTGCAGG GGCATTCCGG TACTGTTGGT AAAGCCACCA TGGAAAAGGT AGGTTCAACC
     1981 ACTGATGCCT AGGCACACCG AAACGACTAA CCCTAATTCT TATCCTTTAC TTCAGGCCAC
     2041 TTAAGcatgg ctagcaaagg agaagaactc ttcactggag ttgtcccaat tcttgttgaa
     2101 ttagatggtg atgttaacgg ccacaagttc tctgtcagtg gagagggtga aggtgatgca
     2161 acatacggaa aacttaccct gaagttcatc tgcactactg gcaaactgcc tgttccctgg
     2221 ccaacactag tctgatctag agtcggggcG GCCGGccgct tcgagcagac atgataagat
     2281 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2341 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2401 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2461 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgaccgat gccctCAAGC
     2521 TTtgagagGG GCCcccttca acccagtcag ctccGACGTC ttggCgccCC cgcggggcat
     2581 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     2641 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2701 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2761 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2821 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2881 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2941 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3001 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3061 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3121 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3181 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3241 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3301 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3361 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3481 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3541 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3601 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3661 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3721 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3781 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3841 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3901 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3961 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4021 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4081 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4141 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4201 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4261 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4321 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4381 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4441 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4501 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4561 ttccccgaaa agtgccacct g
//

