LOCUS pSTAP-seq_fly-zf 4582 bp DNA linear 17-JAN-2017 FEATURES Location/Qualifiers rep_origin 1..456 /dnas_title="f1 origin" /vntifkey="33" /label=f1 origin PCR_primer 689..708 /dnas_title="RVprimer3 binding region" /vntifkey="28" /label=RVprimer3 binding region /current=0 polyA_signal 587..740 /dnas_title="Synthetic (upstream) poly (A)signal" /vntifkey="25" /label=Synthetic (upstream) poly (A)signal misc_feature 1967..2037 /note="MHC intron 16" misc_feature 1970..2036 /note="intron spiced out" polyA_signal 2267..2488 /dnas_title="SV40 late poly (A) signal" /vntifkey="25" /label=SV40 late poly (A) signal misc_marker complement(3591..4451) /dnas_title="Beta-lactamase gene (Ampr)" /vntifkey="22" /label=Beta-lactamase gene (Ampr) misc_feature 2048..2236 /note="truncated sgGFP" primer_bind 2570..2591 /dnas_title="RVprimer4 binding site" /vntifkey="28" /label=RVprimer4 binding site rep_origin 2829..2829 /dnas_title="ColE1-derived plasmid replication origin" /vntifkey="33" /label=ColE1-derived plasmid replication origin rep_origin 2817..3436 /note="pBR322 rep_ori" intron 753..1614 /note="Zfh1 enhancer" enhancer 1909..1923 /note="homology arm IF" PCR_primer complement(1875..1908) /note="illumina adaptor rv" PCR_primer 1671..1703 /note="illumina adapter fw" enhancer 1656..1670 /note="homology arm IF" misc_feature 1704..1874 /note="dpse ush-2 promoter" source 1..4582 /dnas_title="pSTAP-seq_fly-zfh1_spikeIn-4" ORIGIN 1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca 481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat 541 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt 601 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct 661 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc 721 aggtgccaga acatttctct atcgataggt acCTTAAAAG TGCCAGTGCT TGTGGCGTGC 781 ACGGAGAAAA AAACCTTGAG GTCATATTTG AAAAAAAAAA AAAAAAACTA AATTCCAAGT 841 GAGTAGTTTA CTCTTAGTCA CTATCGAGTG TGTGCTGTTT GTTTTTCGAA ATATGCATAT 901 CTTAGCTACT TGCGTTGCTG TTTTTTTTTT TTTTTTTTTT TTTTTGACGA TTTGTCAACA 961 GGAAATATGC ATAAGGTTGG CTCGGGATCC GGATCGGATG GCATGGGGTT TGATTGGATG 1021 CCGTACTTGG CACCGCACTG CCGTTATCGG TTGGCTCATT TCCCGTTAGC CGCGAACAAA 1081 GCCCACGCAT TGTGCTACGA CCGCCGTGAT GTGTTCAAAG ATCAAGTGCC CCCAGAACCA 1141 TCTCCTGCCA TCCCTGCCAT CCCTGCCCGC CTGCATCCAT CGCGGGCATT CAGATTCCGA 1201 CTGCGCATGA GCAGCGGTCT CCAACACGTA AGCAAGTGGA AGGCGAGGGA TAAGAACAAG 1261 TAACTTCTCT CTTATCGGAC GCTTCCAGGA ATTTCGATCC TCGCACCGCA TTCCGCAGGT 1321 TTAGTCACTG GATTCCACGG CTTTTCGAGC TGATTGTCCC AAGAAACATT ATTTTGGACA 1381 TGTGACTCAC TGACTCAAAG CTATTATCAC TTCTCGGACA TATGCGTGTA TATAAAATAT 1441 TTTTAAACAT TATTAGGTAT ACTATAAACT CTGCCTCATT CATGCCTACA GAACTTCAGC 1501 CTTGACATTC TATATATAGA AACCCTTACT ACGTCTTTAC TCTTTAAATA GTTCTTTGGA 1561 TATTATATTA GCACATTGAT GTGCTTTTTT TTTGGATGGT TGTGTCCAAC TCACggtacc 1621 gagctcttac gcgtgctagc ccgggcTCGA GATCTTAGAG CATGCACCGG ACACTCTTTC 1681 CCTACACGAC GCTCTTCCGA TCTCCGGCCG CCCCAGCAGC GCCTGCCACT GCCACACTGC 1741 CACTGCCTGG TGCCGTGCCG TGCCCCGAGT TTCAGTTTGC CATAGAACGC GGACCGAGCA 1801 GCAAGCGGAC ACTCGACACT CAACACTCGA CACTCGACAA GCGAGAGATA GAGATATAAT 1861 CACGTTCGTC ATACAGATCG GAAGAGCACA CGTCTGAACT CCAGTCACTC GACGAATTCG 1921 GCCCCTGCAG GGGCATTCCG GTACTGTTGG TAAAGCCACC ATGGAAAAGG TAGGTTCAAC 1981 CACTGATGCC TAGGCACACC GAAACGACTA ACCCTAATTC TTATCCTTTA CTTCAGGCCA 2041 CTTAAGcatg gctagcaaag gagaagaact cttcactgga gttgtcccaa ttcttgttga 2101 attagatggt gatgttaacg gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc 2161 aacatacgga aaacttaccc tgaagttcat ctgcactact ggcaaactgc ctgttccctg 2221 gccaacacta gtctgatcta gagtcggggc GGCCGGccgc ttcgagcaga catgataaga 2281 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 2341 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 2401 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 2461 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgaccga tgccctCAAG 2521 CTTtgagagG GGCCcccttc aacccagtca gctccGACGT CttggCgccC Ccgcggggca 2581 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 2641 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2701 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2761 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 3001 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 3061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 3121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 3181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 3241 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 3301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3361 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3421 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3481 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3541 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 3601 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 3661 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 3721 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 3781 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 3841 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3901 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3961 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 4021 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 4081 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 4141 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 4201 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 4261 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 4321 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 4381 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 4441 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 4501 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 4561 tttccccgaa aagtgccacc tg //